Structure of an archaeal mechanosensitive channel in closed state

Mechanical coupling of the multiple structural elements of the large-conductance mechanosensitive channel during expansion

chimera of Large conductance mechanosensitive channel protein and Riboflavin synthase
Large conductance mechanosensitive channel protein,Riboflavin synthase

MGSSHHHHHHSSGLVPRGSHMGLFSEFKEFLYEYKVIPLAIAFIMGIASTALIKSFVDNIIMPIITPFVPGGGWETATVE
LGPIVISWGAFLGELVNFIIIAFAVFIIAKKVLQEEKVEKTKKVGIVDTTFARVDMASIAIKKLKELSPNIKIIRKTVPG
IKDLPVACKKLLEEEGCDIVMALGMPGKAEKDKVCAHEASLGLMLAQLMTNKHIIEVFVHEDEAKDDKELDWLAKRRAEE
HAENVYYLLFKPEYLTRMAGKGLRQGFEDAGPARE

7% PEG4000, 0.4M NH4SCN, 0.1M Citric acid(pH7.3)

mechanosensitive channel, mechanosensation, membrane protein, transport protein

MGLFSEFKEFLYEYKVIPLAIAFIMGIASTALIKSFVDNIIMPIITPFVPGGGWETATVELGPIVISWGAFLGELVNFII
IAFAVFIIAKKVLQEEKVEKK

TKKVGIVDTTFARVDMASIAIKKLKELSPNIKIIRKTVPGIKDLPVACKKLLEEEGCDIVMALGMPGKAEKDKVCAHEAS
LGLMLAQLMTNKHIIEVFVHEDEAKDDKELDWLAKRRAEEHAENVYYLLFKPEYLTRMAGKGLRQGFEDAGPARE