Ensemble refinement of cystathione gamma lyase (CalE6) D7G from Micromonospora echinospora

Enzyme Discovery for Natural Product Biosynthesis (NatPro)

(2S)-2-amino-6-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]hexanoic acid

The crystal structure of cystathione gamma lyase (CalE6) from Micromonospora echinospora

SNA(MSE)SG(MSE)RFGTRLVHGGRRPSAGTGDVVPPIHVSTTYERRAQDEPRYFYGRGENPTREELEECLAGLERAPF
ATVFSSGQAAAATLLSLVRPGQCVVSTDDVYAGTDGLFDLAARQGVRVRYADLTTPEGIAAALAEPDLALVWIETPTNPL
LTVVDVAEVSRRAHERGARVVVDNTFASPVLQQPLALGADVSLYSTT(LLP)SIAGHADVLGGALVYRDADLHAAVRAYR
TTAGNVPGALDCFLVRRGLHTLSLRVHRQVATARVLVERLRASPVVGAVHYPGLPEHPQHAVVKAQ(MSE)SAPGAIVSF
DYLGGPAERLLDRFTLFTCGVSLGGVHSLVECPAL(MSE)THRPLSAEARARRGIGESLIRLSVGIEDPQDLAEDLSRAL
AGGT

SNAMSGMRFGTRLVHGGRRPSAGTGDVVPPIHVSTTYERRAQDEPRYFYGRGENPTREELEECLAGLERAPFATVFSSGQ
AAAATLLSLVRPGQCVVSTDDVYAGTDGLFDLAARQGVRVRYADLTTPEGIAAALAEPDLALVWIETPTNPLLTVVDVAE
VSRRAHERGARVVVDNTFASPVLQQPLALGADVSLYSTTKSIAGHADVLGGALVYRDADLHAAVRAYRTTAGNVPGALDC
FLVRRGLHTLSLRVHRQVATARVLVERLRASPVVGAVHYPGLPEHPQHAVVKAQMSAPGAIVSFDYLGGPAERLLDRFTL
FTCGVSLGGVHSLVECPALMTHRPLSAEARARRGIGESLIRLSVGIEDPQDLAEDLSRALAGGT

0.1M MES, 12%(w/v)20,000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K

Enzyme Discovery for Natural Product Biosynthesis

NATURAL PRODUCT BIOSYNTHESIS, NATPRO, LYASE, Structural Genomics, PSI-Biology, Enzyme Discovery for Natural Product Biosynthesis

MSGMRFDTRLVHGGRRPSAGTGDVVPPIHVSTTYERRAQDEPRYFYGRGENPTREELEECLAGLERAPFATVFSSGQAAA
ATLLSLVRPGQCVVSTDDVYAGTDGLFDLAARQGVRVRYADLTTPEGIAAALAEPDLALVWIETPTNPLLTVVDVAEVSR
RAHERGARVVVDNTFASPVLQQPLALGADVSLYSTTKSIAGHADVLGGALVYRDADLHAAVRAYRTTAGNVPGALDCFLV
RRGLHTLSLRVHRQVATARVLVERLRASPVVGAVHYPGLPEHPQHAVVKAQMSAPGAIVSFDYLGGPAERLLDRFTLFTC
GVSLGGVHSLVECPALMTHRPLSAEARARRGIGESLIRLSVGIEDPQDLAEDLSRALAGGT