Structure ALDH7A1 inactivated by 4-diethylaminobenzaldehyde

Diethylaminobenzaldehyde Is a Covalent, Irreversible Inactivator of ALDH7A1.

Cys302 is covalently modified by the attachment of 4-diethylaminobenzaldehyde in a thioacyl linkage.
Alpha-aminoadipic semialdehyde dehydrogenase

Alpha-AASA dehydrogenase,Aldehyde dehydrogenase family 7 member A1,Antiquitin-1,Betaine aldehyde dehydrogenase,Delta1-piperideine-6-carboxylate dehydrogenase,P6c dehydrogenase

GHMSTLLINQPQYAWLKELGLREENEGVYNGSWGGRGEVITTYCPANNEPIARVRQASVADYEETVKKAREAWKIWADIP
APKRGEIVRQIGDALREKIQVLGSLVSLEMGKILVEGVGEVQEYVDICDYAVGLSRMIGGPILPSERSGHALIEQWNPVG
LVGIITAFNFPVAVYGWNNAIAMICGNVCLWKGAPTTSLISVAVTKIIAKVLEDNKLPGAICSLTCGGADIGTAMAKDER
VNLLSFTGSTQVGKQVGLMVQERFGRSLLELGGNNAIIAFEDADLSLVVPSALFAAVGTAGQRCTTARRLFIHESIHDEV
VNRLKKAYAQIRVGNPWDPNVLYGPLHTKQAVSMFLGAVEEAKKEGGTVVYGGKVMDRPGNYVEPTIVTGLGHDASIAHT
ETFAPILYVFKFKNEEEVFAWNNEVKQGLSSSIFTKDLGRIFRWLGPKGSDCGIVNVNIPTSGAEIGGAFGGEKHTGGGR
ESGSDAWKQYMRRSTCTINYSKDLPLAQGIKFQ

Reservoir contained 0.2 M MgCl2, 25% (w/v) PEG3350, 0.1 M Tris at pH of 8.2, 1% DMSO, 200 micromolar 4-diethylaminobenzaldehyde. Protein stock solution contained 3 mg/mL ALDH7A1 and 200 micromolar DEAB.

ALDEHYDE DEHYDROGENASE, NAD, OXIDOREDUCTASE, LYSINE CATABOLISM, oxidoreductase-oxidoreductase inhibitor complex

MSTLLINQPQYAWLKELGLREENEGVYNGSWGGRGEVITTYCPANNEPIARVRQASVADYEETVKKAREAWKIWADIPAP
KRGEIVRQIGDALREKIQVLGSLVSLEMGKILVEGVGEVQEYVDICDYAVGLSRMIGGPILPSERSGHALIEQWNPVGLV
GIITAFNFPVAVYGWNNAIAMICGNVCLWKGAPTTSLISVAVTKIIAKVLEDNKLPGAICSLTCGGADIGTAMAKDERVN
LLSFTGSTQVGKQVGLMVQERFGRSLLELGGNNAIIAFEDADLSLVVPSALFAAVGTAGQRCTTARRLFIHESIHDEVVN
RLKKAYAQIRVGNPWDPNVLYGPLHTKQAVSMFLGAVEEAKKEGGTVVYGGKVMDRPGNYVEPTIVTGLGHDASIAHTET
FAPILYVFKFKNEEEVFAWNNEVKQGLSSSIFTKDLGRIFRWLGPKGSDCGIVNVNIPTSGAEIGGAFGGEKHTGGGRES
GSDAWKQYMRRSTCTINYSKDLPLAQGIKFQ