Crystal structure of binary complex Gon7-Pcc1

Crystal structures of the Gon7/Pcc1 and Bud32/Cgi121 complexes provide a model for the complete yeast KEOPS complex.

Polarized growth chromatin-associated controller 1

Low-dye-binding protein 6,Polarized growth chromatin-associated controller 2

(MSE)TSKREKSLDHTLELKIPFETERQATIATKVLSPDPILKPQDFQVDYSSEKNV(MSE)LVQFRSIDDRVLRVGVSS
IIDSIKTIVEA(MSE)DVLSHHHHHH

MTSKREKSLDHTLELKIPFETERQATIATKVLSPDPILKPQDFQVDYSSEKNVMLVQFRSIDDRVLRVGVSSIIDSIKTI
VEAMDVLSHHHHHH

(MSE)KLPVAQYSAPDGVEKSFAPIRDDPRY(MSE)TTEGRTTGPSDHVLNAGQIDRDKPSEPERTKDGSQLTYLGQLRT
QLTGLQDDINEFLTGR(MSE)ELAKNKKKAGADEKRIQEEINQLLDGGDGDEDAV

MKLPVAQYSAPDGVEKSFAPIRDDPRYMTTEGRTTGPSDHVLNAGQIDRDKPSEPERTKDGSQLTYLGQLRTQLTGLQDD
INEFLTGRMELAKNKKKAGADEKRIQEEINQLLDGGDGDEDAV

Escherichia coli 'BL21-Gold(DE3)pLysS AG'

0.1 M Sodium Acetate pH 6.5, 20% PEG1500, 20% Glycerol, 3% Methanol

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

MTSKREKSLDHTLELKIPFETERQATIATKVLSPDPILKPQDFQVDYSSEKNVMLVQFRSIDDRVLRVGVSSIIDSIKTI
VEAMDVLS