Crystal structure of USP7 ubiquitin-like domains in extended conformation

Crystal Structure of USP7 Ubiquitin-like Domains with an ICP0 Peptide Reveals a Novel Mechanism Used by Viral and Cellular Proteins to Target USP7.

ubiquitin-like domain (UNP residues 535-888)

Deubiquitinating enzyme 7,Herpesvirus-associated ubiquitin-specific protease,Ubiquitin thioesterase 7,Ubiquitin-specific-processing protease 7

(MSE)GSSHHHHHHSSGRENLYFQGDIPQQLVERLQEEKRIEAQKRKERQEAHLY(MSE)QVQIVAEDQFCGHQGND
(MSE)YDEEKVKYTVFKVLKNSSLAEFVQSLSQT(MSE)GFPQDQIRLWP(MSE)QARSNGTKRPA(MSE)LDNEADGNK
T(MSE)IELSDNENPWTIFLETVDPELAASGATLPKFDKDHDV(MSE)LFLK(MSE)YDPKTRSLNYCGHIYTPISCKIR
DLLPV(MSE)CDRAGFIQDTSLILYEEVKPNLTERIQDYDVSLDKALDEL(MSE)DGDIIVFQKDDPENDNSELPTAKEY
FRDLYHRVDVIFCDKTIPNDPGFVVTLSNR(MSE)NYFQVAKTVAQRLNTDP(MSE)LLQFFKSQGYRDGPGNPLRHNYE
GTLRDLLQFFKPRQPKKLYYQQLK(MSE)KITDF

MGSSHHHHHHSSGRENLYFQGDIPQQLVERLQEEKRIEAQKRKERQEAHLYMQVQIVAEDQFCGHQGNDMYDEEKVKYTV
FKVLKNSSLAEFVQSLSQTMGFPQDQIRLWPMQARSNGTKRPAMLDNEADGNKTMIELSDNENPWTIFLETVDPELAASG
ATLPKFDKDHDVMLFLKMYDPKTRSLNYCGHIYTPISCKIRDLLPVMCDRAGFIQDTSLILYEEVKPNLTERIQDYDVSL
DKALDELMDGDIIVFQKDDPENDNSELPTAKEYFRDLYHRVDVIFCDKTIPNDPGFVVTLSNRMNYFQVAKTVAQRLNTD
PMLLQFFKSQGYRDGPGNPLRHNYEGTLRDLLQFFKPRQPKKLYYQQLKMKITDF

sodium chloride, TRIS-HCl, 2-mercaptoethanol, betaine hydrochloride

Canadian Institutes of Health Research (CIHR)

Herpes simplex virus (type 1 / strain 17)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED

deubiquitinating (DUB) enzyme, ubiquitin-like (Ubl) domains, ICP0 binding site, HAUSP, HYDROLASE

DIPQQLVERLQEEKRIEAQKRKERQEAHLYMQVQIVAEDQFCGHQGNDMYDEEKVKYTVFKVLKNSSLAEFVQSLSQTMG
FPQDQIRLWPMQARSNGTKRPAMLDNEADGNKTMIELSDNENPWTIFLETVDPELAASGATLPKFDKDHDVMLFLKMYDP
KTRSLNYCGHIYTPISCKIRDLLPVMCDRAGFIQDTSLILYEEVKPNLTERIQDYDVSLDKALDELMDGDIIVFQKDDPE
NDNSELPTAKEYFRDLYHRVDVIFCDKTIPNDPGFVVTLSNRMNYFQVAKTVAQRLNTDPMLLQFFKSQGYRDGPGNPLR
HNYEGTLRDLLQFFKPRQPKKLYYQQLKMKITDF