Crystal Structure of Full-Length Split GFP Mutant D21H/K26C Disulfide and Metal-Mediated Dimer, P 21 21 21 Space Group, Form 3

{2-[(1R,2R)-1-amino-2-hydroxypropyl]-4-(4-hydroxybenzylidene)-5-oxo-4,5-dihydro-1H-imidazol-1-yl}acetic acid

A Suite of Engineered GFP Molecules for Oligomeric Scaffolding.

GMRKGEELFTGVVPILIELDGHVNGHCFFVRGEGEGDATIGKLSLKFIATTGKLPVPWPTLVTTL(CRO)VQCFSRYPDH
MKRHDFFKSAMPEGYVQERTIYFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHKVYITADKQ
NNGIKANFTIRHNVEDGSVQLADHYQQNTPIGDGPVDLPDDHYLSTQTILSKDLNEKRDHMVLLEYVTAAGIT

GMRKGEELFTGVVPILIELDGHVNGHCFFVRGEGEGDATIGKLSLKFIATTGKLPVPWPTLVTTLTYGVQCFSRYPDHMK
RHDFFKSAMPEGYVQERTIYFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHKVYITADKQNN
GIKANFTIRHNVEDGSVQLADHYQQNTPIGDGPVDLPDDHYLSTQTILSKDLNEKRDHMVLLEYVTAAGIT

50% PEG200, 0.2M MgCl2, 0.1M NaCacodylate pH 6.5

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)