Crystal structure of anti-FPR Fpro0165 Fab fragment

Affinity Maturation of a Novel Antagonistic Human Monoclonal Antibody with a Long Vh Cdr3 Targeting the Class a Gpcr Formyl-Peptide Receptor 1.

EVQLVESGGGLVKPGGSLRLSCAASGFTFSNAWMNWVRQAPGKGLEWVGRVKSKTDGGAADYAAPVKGRFTISRDDSKNT
LYLQMNSLKTEDTAVYYCTTDQLGYDFWNGYYTDTLYYYFGMDVWGPGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAAL
GCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK
THT

DIVMTQSPLSLPVTPGEPASISCRSSQPLLAMDGHNYLDWYLQKPGQSPQVLIYQSKWRASGVPDRFSGSGSGTDFTLKI
SRVEAEDVGVYYCMQALQTPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQ
SGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

REFINEMENT WAS PERFORMED USIN RESOLUTION FAB FRAGMENT STRUCTURES WITH HIGH HOMOLOGY TO FPR0165 FAB WERE USED TO BUILD A MODEL FOR TARGET RESTRAINTS, PDB CODE 3AAZ AND PDB CODE 3GHB FOR THE LIGHT AND HEAVY CHAINS RESPECTIVELY.

IMMUNE SYSTEM, ANTIBODY ENGINEERING, PHAGE DISPLAY, LONG CDR, FORMYL PEPTIDE RECEPTOR-1