Crystal structure determination of uracil-DNA N-glycosylase (UNG) from Deinococcus radiodurans in complex with DNA - new insights into the role of the Leucine-loop for damage recognition and repair

Structure determination of uracil-DNA N-glycosylase from Deinococcus radiodurans in complex with DNA.

RESIDUES 1-15 AND 246-247 NOT VISIBLE IN ELECTRON DENSITY

MTDQPDLFGLAPDAPRPIIPANLPEDWQEALLPEFSAPYFHELTDFLRQERKEYTIYPPAPDVFNALRYTPLGEVKVLIL
GQDPYHGPNQAHGLSFSVRPGVRVPPSLRNIYKELTEDIPGFVAPKHGYLRSWAEQGVLLLNAVLTVRAAQANSHQGKGW
EHFTDAVIKAVNAKEERVVFILWGSYARKKKKLITGKNHVVIESGHPSPLSEQYFFGTRPFSKTNEALEKAGRGPVEWQL
PATVTEE

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES WITH TLS ADDED TERMINAL DNA NUCLEOTIDES HAD HIGH B-FACTORS, BUT WERE VISIBLE IN ELECTRON DENSITY CONTOURED AT LOW SIGMA LEVEL.

HYDROLASE-DNA COMPLEX, BASE EXCISION REPAIR, RADIATION RESISTANCE, DNA DAMAGE, DNA REPAIR, PROTEIN-DNA COMPLEX