A new bio-isosteric base pair based on reversible bonding

[(2R,3S,5R)-3-hydroxy-5-(3-hydroxy-4-methanoyl-phenyl)oxolan-2-yl]methyl dihydrogen phosphate

"Post-it" type connected DNA created with a reversible covalent cross-link.

5'-D(*GP*AP*CP*C T0TP*TP*CP*CP*CP*TP)-3'

5'-D(*AP*GP*GP*GP*A SAYP*GP*GP*TP*CP)-3'

KMAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVGIAVVNEHGRFFLRPETALADPQFVAWLGDETKKKSMFDSKRAA
VALKWKGIELCGVSFDLLLAAYLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEHLVRKAAAIWAL
ERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVDTKRLEQMGEELAEQLRTVEQRIYELAGQEFNINSPKQLGVI
LFEKLQLPVLKKSKTGYSTSADVLEKLAPYHEIVENILHYRQLGKLQSTYIEGLLKVVRPDTKKVHTIFNQALTQTGRLS
STEPNLQNIPIRLEEGRKIRQAFVPSESDWLIFAADYSQIELRVLAHIAEDDNLMEAFRRDLDIHTKTAMDIFQVSEDEV
TPNMRRQAKAVNFGIVYGISDYGLAQNLNISRKEAAEFIERYFESFPGVKRYMENIVQEAKQKGYVTTLLHRRRYLPDIT
SRNFNVRSFAERMAMNTPIQGSAADIIKKAMIDLNARLKEERLQARLLLQVHDELILEAPKEEMERLCRLVPEVMEQAVT
LRVPLKVDYHYGSTWYDAK

GERMAN COLLECTION OF MICROORGANISMS (DSM)

46-49% AMMONIUM SULFATE, 2-3% MPD, 100MM MES PH5.8

oligosaccharide with reducing-end-to-reducing-end glycosidic bond

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

TRANSFERASE-DNA COMPLEX, UNNATURAL BASE PAIR