CRYSTAL STRUCTURE OF A ZINC-TRANSPORTING PIB-TYPE ATPASE IN E2.PI STATE

Structure and Mechanism of Zn(2+)-Transporting P-Type Atpases.

MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSENVSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQ
VLFATEKLVVDADNDIRAQVESAVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEGWAASRARQGVSALMALKPETAT
RLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLLSPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSE
PGASAIDRILKLIEEAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGCPCALVIST
PAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTAIHPATGISESELLTLAAAVEQGATHPLAQA
IVREAQVAELAIPTAESQRALVGSGIEAQVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDT
LRADAATAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLAMVGDGINDAPAMKAAA
IGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANIRQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVL
VTANALRLLRRR

300 MM LITHIUM ACETATE, 3% V/V T-BUOH, 14 % POLY ETHYLENE GLYCOL 2000 MONOMETHYL ETHER (PEG2000-MME), 7 % V/V SORBITOL,10 % V/V GLYCEROL AND 5 MM BME

CRYSTAL STRUCTURE OF A ZINC-TRANSPORTING PIB-TYPE ATPASE IN THE E2P STATE

CPC, CXXC, ATP-BINDING, HYDROLASE, ION TRANSPORT, MAGNESIUM, ZN2+, METAL-BINDING, NUCLEOTIDE-BINDING, TRANSMEMBRANE, TRANSPORT, HEAVY-METAL BINDING, P-TYPE ATPASE, PIB-ATPASE, ZN2+ EXPORTING, ZINC TRANSPORT, PI-ATPASE, MEMBRANE PROTEIN