Crystal structure of Argininosuccinate synthase from Mycobacterium thermoresistibile

Seattle Structural Genomics Center for Infectious Disease (SSGCID)

MAHHHHHHMSERVILAYSGGLDTSVAISWIGKETGREVVAVAIDLGQGGEDMEVVRQRALDCGAVESIVIDARDEFANDY
CVPAIQSNALYMDRYPLVSALSRPLIVKHLVKAAREHGGTIVAHGCTGKGNDQVRFEVGFASLAPDLEVLAPVRDYAWTR
EKAIAFAEENNIPINVTKRSPFSIDQNVWGRAVETGFLEHLWNAPTKDVYSYTEDPTVNWSTPDEVIVGFEQGVPVSIDG
RSVTPLQAIEELNRRGGEQGVGRLDVVEDRLVGIKSREIYEAPGAMVLITAHTELEHVTLERELGRFKRITDQKWGELVY
DGLWFSPLKTALESFVAKTQEHVTGEIRMVLHGGHIAVNGRRSPKSLYDFNLATYDEGDTFDQSAAKGFVQIHGLSSSIS
ARRDLQGQ

RigakuReagents JCSG+ screen, D6: 20% PEG 8000, 200mM MgCl2, 100mM Tris/HCl pH 8.5; cryo 20% EG; MythA.00809.a.B1.PW37508 at 30.1mg/ml, tray 256225d6, puck zrb0-6

Seattle Structural Genomics Center for Infectious Disease
NIAID, National Institute of Allergy and Infectious Diseases

1kh1 modified with CCP4 program CHAINSAW

SSGCID, Argininosuccinate synthase, Citrulline-aspartate ligase, Mycobacterium thermoresistibile, Structural Genomics, Seattle Structural Genomics Center for Infectious Disease, LIGASE

MSERVILAYSGGLDTSVAISWIGKETGREVVAVAIDLGQGGEDMEVVRQRALDCGAVESIVIDARDEFANDYCVPAIQSN
ALYMDRYPLVSALSRPLIVKHLVKAAREHGGTIVAHGCTGKGNDQVRFEVGFASLAPDLEVLAPVRDYAWTREKAIAFAE
ENNIPINVTKRSPFSIDQNVWGRAVETGFLEHLWNAPTKDVYSYTEDPTVNWSTPDEVIVGFEQGVPVSIDGRSVTPLQA
IEELNRRGGEQGVGRLDVVEDRLVGIKSREIYEAPGAMVLITAHTELEHVTLERELGRFKRITDQKWGELVYDGLWFSPL
KTALESFVAKTQEHVTGEIRMVLHGGHIAVNGRRSPKSLYDFNLATYDEGDTFDQSAAKGFVQIHGLSSSISARRDLQGQ