Crystal structure of dienelactone hydrolase A-6 variant (S7T, A24V, Q35H, F38L, Q110L, C123S, Y145C, E199G and S208G) at 1.95 A resolution

S-CYSTEINESULFINIC ACID; S-SULFINOCYSTEINE

Directed evolution of new and improved enzyme functions using an evolutionary intermediate and multidirectional search.

MLTEGITIQSYDGHTFGALVGSPVKAPAPVIVIAHEILGVNAFMRETVSWLVDQGYAAVCPDLYARQAPGTALDPQDEAQ
REQAYKLWQAFDMEAGVGDLEAAIRYARHLPYSNGKVGLVGYSLGGALAFLVAAKGYVDRAVGY(CSD)GVGLEKQLNKV
PEVKHPALFHMGGQDHFVPAPSRQLITEGFGANPLLQVHWYEGAGHSFARTGSSGYVASAAALANERTLDFLAPLQSKKP

MLTEGITIQSYDGHTFGALVGSPVKAPAPVIVIAHEILGVNAFMRETVSWLVDQGYAAVCPDLYARQAPGTALDPQDEAQ
REQAYKLWQAFDMEAGVGDLEAAIRYARHLPYSNGKVGLVGYSLGGALAFLVAAKGYVDRAVGYCGVGLEKQLNKVPEVK
HPALFHMGGQDHFVPAPSRQLITEGFGANPLLQVHWYEGAGHSFARTGSSGYVASAAALANERTLDFLAPLQSKKP

HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT

MLTEGISIQSYDGHTFGALVGSPAKAPAPVIVIAQEIFGVNAFMRETVSWLVDQGYAAVCPDLYARQAPGTALDPQDERQ
REQAYKLWQAFDMEAGVGDLEAAIRYARHQPYSNGKVGLVGYCLGGALAFLVAAKGYVDRAVGYYGVGLEKQLKKVPEVK
HPALFHMGGQDHFVPAPSRQLITEGFGANPLLQVHWYEEAGHSFARTSSSGYVASAAALANERRLDFLAPLQSKKP