Crystal structure of double mutant E. Coli purine nucleoside phosphorylase with 4 FMC molecules

Crystallographic snapshots of ligand binding to hexameric purine nucleoside phosphorylase and kinetic studies give insight into the mechanism of catalysis.

Purine nucleoside phosphorylase DeoD-type

ATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDF
GVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFY
SPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSAHIRTHEQTTAAEAQTTFNDMIKIALESVLLGDK

50 mM citric buffer, 34 % ammonium sulphate

The biological assembly is a hexamer. One hexamer is found in asymmetric unit.

Purine nucleoside phosphorylase, formicyn A, transferase

ATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDF
GVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFY
SPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDK