Crystal structure of 6-phosphogluconolactonase from Mycobacterium smegmatis N131D mutant soaked with CuSO4

Enzyme repurposing of a hydrolase as an emergent peroxidase upon metal binding.

ASWSHPQFEKGAMSDTVIERHADTAALVAAAGDRLVDAISSAIGERGQATIVLTGGGTGIGLLKRVRERSGEIDWSKVHI
YWGDERFVPQDDDERNDKQAREALLDHIGIPPVNVHAMAASDGEFGDDLEAAAAGYAQLLSADFDSSVPGFDVHLLGMGG
EGHVNSLFPDTDAVRETERLVVGVSDSPKPPPRRITLTLPAVQNSREVWLVVSGEAKADAVAAAVGGADPVDIPAAGAVG
RERTVWLVDEAAAAKL

PARTLY OCCUPIED COPPER IONS (CU1 305 - CU 320) WERE IDENTIFIED BY ANOMALOUS DIFFERENCE DENSITY

MSDTVIERHADTAALVAAAGDRLVDAISSAIGERGQATIVLTGGGTGIGLLKRVRERSGEIDWSKVHIYWGDERFVPQDD
DERNDKQAREALLDHIGIPPVNVHAMAASDGEFGDDLEAAAAGYAQLLSANFDSSVPGFDVHLLGMGGEGHVNSLFPDTD
AVRETERLVVGVSDSPKPPPRRITLTLPAVQNSREVWLVVSGEAKADAVAAAVGGADPVDIPAAGAVGRERTVWLVDEAA
AAKL