Crystal structure of the Lsd19-lasalocid A complex

6-[(3R,4S,5S,7R)-7-{(2S,3S,5S)-5-ethyl-5-[(2R,5R,6S)-5-ethyl-5-hydroxy-6-methyltetrahydro-2H-pyran-2-yl]-3-methyltetrahydrofuran-2-yl}-4-hydroxy-3,5-dimethyl-6-oxononyl]-2-hydroxy-3-methylbenzoic acid

Epoxide hydrolase-lasalocid a structure provides mechanistic insight into polyether natural product biosynthesis.

GRHMPAETVRKEVALEYCRRVNAGELEGVLQLFAPDARLVDPLGTEPVVGRAALAARLAPALRGAVHEEPGRPYAAHDGT
SVVLPATVTVGAPGAPPQRRGRTRVMGVIEVGEDGLIREMRVMWGVTDSSWTARPAPDEERRKELAREHCLRINDGDVDG
LLKLYSPRIRFEDPVGSWTRTGLEALRAHATMAVGSNVRETAGLTVAGQDGRHAAVTVSATMDYLPSGPLLARHHLMTLP
APADPHRALIGIEYVMVIGVDADGLIDEMRAYWGATDVSLLDP

1.65 M sodium formate, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K

Epoxide hydrolase complexed with a different ligand

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

NTF2-like fold, epoxide-opening cyclic ether formation, isomerization, ISOMERASE

MPAETVRKEVALEYCRRVNAGELEGVLQLFAPDALLVDPLGTEPVVGRAALAARLAPALRGAVHEEPGRPYAAHDGTSVV
LPATVTVGAPGAPPQRRGRTRVMGVIEVGEDGLIREMRVMWGVTDSSWTARPAPDEERRKELAREHCLRINDGDVDGLLK
LYSPRIRFEDPVGSWTRTGLEALRAHATMAVGSNVRETAGLTVAGQDGRHAAVTVSATMDYLPSGPLLARHHLMTLPAPA
DPHRALIGIEYVMVIGVDADGLIDEMRAYWGATDVSLLDP