Crystal structure of tRNA fluorescent labeling enzyme

PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER
ADENOSINE-5'-[BETA, GAMMA-METHYLENE]TRIPHOSPHATE

A covalent approach for site-specific RNA labeling in Mammalian cells.

tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS

tRNA(Ile2)-agm2C synthetase, tRNA(Ile2) agmatidine synthetase

MRVWVGIDDTDSSRGMC(TPO)TYLAVLAMERVERELGKVIGFPRLIRLNPTIPYKTRGNGAVSFLVEVDDVGELVDVVN
EVIIEHAMLDDEKTNPGAVFVDEELAVKLKPFADKAIKDVLQIDEALFVIGKYFIPHLRHKKGRGLIGALAAVGAELEDF
TLELIAYRYPERFGTEREYDEESFFDMDYELYPQTFDNVDWCNDVVVCIPNTPCPVLYGIRGESVEALYKAMESVKTEPV
DRRMIFVTNHATDMHLIGEEEVHRLENYRSYRLRGRVTLEPYDIEGGHVFFEIDTKFGSVKCAAFEPTKQFRNVIRLLRK
GDVVEVYGSMKKDTINLEKIQIVELAEIWVEKNPICPSCGRRMESAGRGQGFRCKKCRTKADEKLREKVERELQPGFYEV
PPSARRHLSKPLIRMNVEGRHIFR

MRVWVGIDDTDSSRGMCTTYLAVLAMERVERELGKVIGFPRLIRLNPTIPYKTRGNGAVSFLVEVDDVGELVDVVNEVII
EHAMLDDEKTNPGAVFVDEELAVKLKPFADKAIKDVLQIDEALFVIGKYFIPHLRHKKGRGLIGALAAVGAELEDFTLEL
IAYRYPERFGTEREYDEESFFDMDYELYPQTFDNVDWCNDVVVCIPNTPCPVLYGIRGESVEALYKAMESVKTEPVDRRM
IFVTNHATDMHLIGEEEVHRLENYRSYRLRGRVTLEPYDIEGGHVFFEIDTKFGSVKCAAFEPTKQFRNVIRLLRKGDVV
EVYGSMKKDTINLEKIQIVELAEIWVEKNPICPSCGRRMESAGRGQGFRCKKCRTKADEKLREKVERELQPGFYEVPPSA
RRHLSKPLIRMNVEGRHIFR

50 mM HEPES, pH 7.0-7.2, 0.5 M NH4Ac, 0.02 M MgCl2, 1.5-2.5% PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 289K

selenomethionine derived Tias coordinate

AUTHORS STATE THAT THE BIOLOGICAL ASSEMBLY IS UNKNOWN.

tRNA modification, phosphorylation, tias, LIGASE