Crystal structure of MtmC in complex with SAM and TDP

Structural Insight into MtmC, a Bifunctional Ketoreductase-Methyltransferase Involved in the Assembly of the Mithramycin Trisaccharide Chain.

MDIYGTARAVTTCRMCGAQDWQEVVDFGPVPLADSFLEPAASYDDEPRYPLAVVSCRSCRLMSLTHVVDPEVLYRTYPYT
TSDSETIKKHMGHVVAVCVERFGIPEGSFVLEIGSNTGSQLKAFQNAGMRTLGIDPARNIAAVANERGIETLPEFFSVDT
AALVKKTHGTPQLVLGRHVFAHIDDVSAVAEGVRDLLGPDSLFAIEVPYLVDMLERNEFDTIYHEHLSYIGVGSLVALFR
RHGLRVVDVERLAVHGGSILVFVGLDEGTRATAPVVEELIALEKERGLYEDATYERFARHVAEITAELTSMVRSLRAEGK
RIAGYGAPAKGNTLLNVCGLTADDLEFCCDTTEFKQGLVLPGTHIPVRSPEYAKTQAIDYYLLLAWNYGEEILAKEGPFL
ADGGRFILPNPRPSIVPPGEHHHHHH

0.1 M MES, pH 5.5, 0.2 M ammonium acetate, 16% PEG 4000, 1 mM TDP, 1 mM SAM, VAPOR DIFFUSION, HANGING DROP, temperature 295K

same protein in complex with SAH and substrate

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

C-methyltransferase, TDP-4-keto-D-olivose, Transferase

MDIYGTARAVTTCPMCGAQDWQEVVDFGPVPLADSFLEPAASYDDEPRYPLAVVSCASCRLMSLTHVVDPEVLYRTYPYT
TSDSETIKKHMGHVVAGVRPSASASPEGSFVLEIGSNTGSQLKAFQNAGMRTLGIDPARNIAAVANERGIETLPEFFSVD
TAALVKKTHGTPQLVLGRHVFAHIDDVSAVAEGVRDLLGPDSLFAIEVPYLVDMLERNEFDTIYHEHLSYIGVGSLVALF
RRHGLRVVDVERLAVHGGSILVFVGLDEGTRATAPVVEELIALEKERGLYEDATYERFARHVAEITAELTSMVRSLRAEG
KRIAGYGAPAKGNTLLNVCGLTADDLEFCCDTTEFKQGLVLPGTHIPVRSPEYAKTQAIDYYLLLAWNYGEEILAKEGPF
LADGGRFILPNPRPSIVPPGE