Crystal Structure of gamma-carotenoid desaturase

Crystal structure of 1'-OH-carotenoid 3,4-desaturase from Nonlabens dokdonensis DSW-6.

MKNAIVIGAGIGGLAAALRLRHQGYSVTIFEKNDYAGGKLHAIEKDGYRFDLGPSLFTLPHLVENLFALFPEEIIDFGYK
SKAISFHYFWDDGTLFKASTDSSQFIEDASKVFKEEKSTIKKYLAKSKSKYELTKSLFLEKSLHKATTYFSLDTVKAIVH
APFLGLNNTLNDENSKFKNPKLTQLFNRYATYNGSSPYQTPGIMTMIPHLELGLGTYYPDGGMHRISQSLFELAQKVGVK
FRFRESVTNITTSKNKVTGVETKNGSYLSDLVVSNMDIVPTYRNLMKDVPAPEKTLSQERSSSALIFYWGIDREFPELDL
HNILFSEDYKTEFEHIFEHKTLAQDPTVYINITSKESSNDAPAGHENWFVMINAPGDYGQDWEQLVEESKKQIIAKIKKC
LHVDISKHITTEYILTPQGIEKNTSSYRGALYGAASNNKFAAFLRHPNFNGKIKNLYHVGGSVHPGGGIPLCLLSAQITA
DLIQKEQLEHHHHHH

14% PEG3350, 0.1 M succinic acid, pH 7.0, 50 mM dimethylbenzylammonium propane sulfonate, VAPOR DIFFUSION, HANGING DROP, temperature 295K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Rossmann fold, FAD-binding, OXIDOREDUCTASE, FLAVOPROTEIN, DESATURASE, gamma-carotenoid desaturase

MKNAIVIGAGIGGLAAALRLRHQGYSVTIFEKNDYAGGKLHAIEKDGYRFDLGPSLFTLPHLVENLFALFPEEIIDFGYK
SKAISFHYFWDDGTLFKASTDSSQFIEDASKVFKEEKSTIKKYLAKSKSKYELTKSLFLEKSLHKATTYFSLDTVKAIVH
APFLGLNNTLNDENSKFKNPKLTQLFNRYATYNGSSPYQTPGIMTMIPHLELGLGTYYPDGGMHRISQSLFELAQKVGVK
FRFRESVTNITTSKNKVTGVETKNGSYLSDLVVSNMDIVPTYRNLMKDVPAPEKTLSQERSSSALIFYWGIDREFPELDL
HNILFSEDYKTEFEHIFEHKTLAQDPTVYINITSKESSNDAPAGHENWFVMINAPGDYGQDWEQLVEESKKQIIAKIKKC
LHVDISKHITTEYILTPQGIEKNTSSYRGALYGAASNNKFAAFLRHPNFNGKIKNLYHVGGSVHPGGGIPLCLLSAQITA
DLIQKEQ