High-resolution crystal structure of Z-DNA in complex with Cr3+ cations
High-resolution crystal structure of Z-DNA in complex with Cr(3+) cations.
Ultrahigh-resolution crystal structures of Z-DNA in complex with Mn(2+) and Zn(2+) ions.
Phosphates in the Z-DNA dodecamer are flexible, but their P-SAD signal is sufficient for structure solution.
High regularity of Z-DNA revealed by ultra high-resolution crystal structure at 0.55 A.
A 1.5 MM WATER SOLUTION OF DNA MIXED 1:1 V/V WITH 10% MPD, 12 MM SPERMINE*4HCL, 12 MM NACL, 80 MM KCL AND EQUILIBRATED AGAINST 35% MPD, PH 6. FOR CR3+ SOAKING, THE CRYSTAL WAS PLACED FOR SEVERAL DAYS IN 0.002 ML OF THE RESERVOIR SOLUTION MIXED WITH 0.002 ML OF 5 MM [CR(H2O)6]CL3, VAPOR DIFFUSION, HANGING DROP, temperature 292.0K
THE REFINEMENT WAS CARRIED OUT AGAINST SEPARATE BIJVOET PAIRS. ANISOTROPIC ATOMIC DISPLACEMENT PARAMETERS WERE USED. ANISOTROPIC REFINEMENT REDUCED FREE R-FACTOR FROM 0.2585 TO 0.1976. HYDROGEN ATOMS WERE ADDED AT RIDING POSITION THE FINAL REFINEMENT WAS CALCULATED USING WEIGHTED FULL-MATRIX LEAST-SQUARES PROCEDURE AND ALL REFLECTIONS.
PHOSPHATE AND GLYCOSIDIC ANGLES ACCORDING TO PDB MODEL 3P4J
MOEWS & KRETSINGER, J.MOL.BIOL.(1975) 91, 201
Self-complementary dimer of chains A and B