Cry3A Toxin structure obtained by Serial Femtosecond Crystallography from in vivo grown crystals isolated from Bacillus thuringiensis and data processed with the cctbx.xfel software suite

Protein crystal structure obtained at 2.9 angstrom resolution from injecting bacterial cells into an X-ray free-electron laser beam.

Cornell-SLAC Pixel Array Detector (CSPAD)

no monochromator, FEL beam with 20-30 eV bandwidth

73 kDa crystal protein, Crystaline entomocidal protoxin, Insecticidal delta-endotoxin CryIIIA(a), delta endotoxin Cry3A

DSSTTKDVIQKGISVVGDLLGVVGFPFGGALVSFYTNFLNTIWPSEDPWKAFMEQVEALMDQKIADYAKNKALAELQGLQ
NNVEDYVSALSSWQKNPVSSRNPHSQGRIRELFSQAESHFRNSMPSFAISGYEVLFLTTYAQAANTHLFLLKDAQIYGEE
WGYEKEDIAEFYKRQLKLTQEYTDHCVKWYNVGLDKLRGSSYESWVNFNRYRREMTLTVLDLIALFPLYDVRLYPKEVKT
ELTRDVLTDPIVGVNNLRGYGTTFSNIENYIRKPHLFDYLHRIQFHTRFQPGYYGNDSFNYWSGNYVSTRPSIGSNDIIT
SPFYGNKSSEPVQNLEFNGEKVYRAVANTNLAVWPSAVYSGVTKVEFSQYNDQTDEASTQTYDSKRNVGAVSWDSIDQLP
PETTDEPLEKGYSHQLNYVMCFLMQGSRGTIPVLTWTHKSVDFFNMIDSKKITQLPLVKAYKLQSGASVVAGPRFTGGDI
IQCTENGSAATIYVTPDVSYSQKYRARIHYASTSQITFTLSLDGAPFNQYYFDKTINKGDTLTYNSFNLASFSTPFELSG
NNLQIGVTGLSAGDKVYIDKIEFIPVN

bt13, cry3A, cry3Aa, cryC, cryIIIa, cryIIIA(a)
Bacillus thuringiensis serovar tenebrionis
Bacillus thuringiensis subsp. israelensis

Cry3A crystallized within the bacterial cell, IN VIVO CRYSTALLIZATION, temperature 303K

Structure of Cry3A Toxin, crystals isolated from the cell, data processed using CrystFEL

Structure of Cry3A Toxin, data collected by injecting cells in the beam, data processed using cctbx.xfel

Structure of Cry3A Toxin, data collected by injecting cells in the beam, data processed using CrystFEL

in vivo crystals, microcrystals, serial femtosecond crystallography, SFX, LCLS, X-ray Free-electron laser, insecticidal toxin, TOXIN