CRYSTAL STRUCTURE OF PSPF(1-265) E108Q MUTANT bound to ATP

4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID

Molecular basis of nucleotide-dependent substrate engagement and remodeling by an AAA+ activator.

KIRKPATRICK BAEZ BIMORPH MIRROR PAIR FOR HORIZONTAL AND VERTICAL FOCUSSING

Phage Shock protein F AAA DOMAIN, RESIDUES 1-265

MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKELIASRLHYLSSRWQGPFISLNCAALNENLLDSELFGH
EAGAFTGAQKRHPGRFERADGGTLFLDQLATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
FRADLLDRLAFDVVQLPPLRERESDIMLMAEYFAIQMCREIKLPLFPGFTERARETLLNYRWPGNIRELKNVVERSVYRH
GTSDYPLDDIIIDPFKRRPPEDAIA

100mM Bis-Tris pH 8.0, 12-16% MPD, 2M Ammonium formate , VAPOR DIFFUSION, SITTING DROP, temperature 292K

CRYSTAL STRUCTURE OF PSPF(1-275) R168A MUTANT

AAA DOMAIN, RESIDUES 1-265 bound to AMPPNP

AAA domain, Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene, BACTERIAL SIGMA54 ACTIVATOR, ATP Binding, DNA binding, TRANSCRIPTION

MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKELIASRLHYLSSRWQGPFISLNCAALNENLLDSELFGH
EAGAFTGAQKRHPGRFERADGGTLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
FRADLLDRLAFDVVQLPPLRERESDIMLMAEYFAIQMCREIKLPLFPGFTERARETLLNYRWPGNIRELKNVVERSVYRH
GTSDYPLDDIIIDPFKRRPPEDAIA