Crystal structure of AAA+sigma 54 activator domain of the flagellar regulatory protein FlrC of Vibrio cholerae in nucleotide free state

Unique ATPase site architecture triggers cis-mediated synchronized ATP binding in heptameric AAA+-ATPase domain of flagellar regulatory protein FlrC

HHHHHHSSGLVPRGSHMVVADTKSLKLLALADKVAKTDANVMILGPSGSGKEVMSRYIHNASPRKEGPFIAINCAAIPDN
MLEATLFGYEKGAFTGAVQACPGKFEQAQGGTILLDEISEMDLNLQAKLLRVLQEREVERLGSRKSIKLDVRVLATSNRD
LKQYVQAGHFREDLYYRLNVFPLTWPALCERKDDIEPLANHLIERHCKKLGLPVPSIAPNAITKLLNYPWPGNVRELDNV
VQRALILSENGHIQSEHILLEGVDWHD

10% PEG 6000, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

AAA+ ATPase domain, ATPase activity, TRANSCRIPTION

VVADTKSLKLLALADKVAKTDANVMILGPSGSGKEVMSRYIHNASPRKEGPFIAINCAAIPDNMLEATLFGYEKGAFTGA
VQACPGKFEQAQGGTILLDEISEMDLNLQAKLLRVLQEREVERLGSRKSIKLDVRVLATSNRDLKQYVQAGHFREDLYYR
LNVFPLTWPALCERKDDIEPLANHLIERHCKKLGLPVPSIAPNAITKLLNYPWPGNVRELDNVVQRALILSENGHIQSEH
ILLEGVDWHD