3.1 A resolution crystal structure of the B. pertussis BvgS periplasmic domain

Virulence Regulation with Venus Flytrap Domains: Structure and Function of the Periplasmic Moiety of the Sensor-Kinase BvgS.

MQYKLALNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTELVPRGSAQASQELTLVGKAAVPDV
EVALDGDDWRWLARKRVLTLGVYAPDIPPFDVTYGERYEGLTADYMAIIAHNLGMQAKVLRYPTREQALSALESGQIDLI
GTVNGTDGRQQSLRLSVPYAADHPVIVMPIGARHVPASNLAGQRLAVDINYLPKETLARAYPQATLHYFPSSEQALAAVA
YGQADVFIGDALTTSHLVSQSYFNDVRVVAPAHIATGGESFGVRADNTRLLRVVNAVLEAIPPSEHRSLIYRWGLGSSIS
LDFAHPAYSAREQQWMADHPVVKVAVLNLFAPFTLFRTDEQFGGISAAVLQLLQLRTGLDFEIIGVDTVEELIAKLRSGE
ADMAGALFVNSARESFLSFSRPYVRNGMVIVTRQDPDAPVDADHLDGRTVALVRNSAAIPLLQRRYPQAKVVTADNPSEA
MLMVANGQADAVVQTQISASYYVNRYFAGKLRIASALDLPPAEIALATTRGQTELMSILNKALYSISNDELASIISRWRG
SDGDPRTWYAYRNEIYLEHHHHHH

N-terminal GB1-tag and C-terminal His-tag

100 mM Sodium Acetate (pH 4.6), 1.6 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Bacterial extracellular solute-binding proteins, family 3, venus fly trap, periplasmic binding protein, sensor domain, virulence regulation, environmental signals, signal perception, TRANSFERASE

AQASQELTLVGKAAVPDVEVALDGDDWRWLARKRVLTLGVYAPDIPPFDVTYGERYEGLTADYMAIIAHNLGMQAKVLRY
PTREQALSALESGQIDLIGTVNGTDGRQQSLRLSVPYAADHPVIVMPIGARHVPASNLAGQRLAVDINYLPKETLARAYP
QATLHYFPSSEQALAAVAYGQADVFIGDALTTSHLVSQSYFNDVRVVAPAHIATGGESFGVRADNTRLLRVVNAVLEAIP
PSEHRSLIYRWGLGSSISLDFAHPAYSAREQQWMADHPVVKVAVLNLFAPFTLFRTDEQFGGISAAVLQLLQLRTGLDFE
IIGVDTVEELIAKLRSGEADMAGALFVNSARESFLSFSRPYVRNGMVIVTRQDPDAPVDADHLDGRTVALVRNSAAIPLL
QRRYPQAKVVTADNPSEAMLMVANGQADAVVQTQISASYYVNRYFAGKLRIASALDLPPAEIALATTRGQTELMSILNKA
LYSISNDELASIISRWRGSDGDPRTWYAYRNEIYL