Crystal structure of D-glucuronyl C5-epimerase in complex with heparin hexasaccharide

O2-SULFO-GLUCURONIC ACID; 2-O-sulfo-alpha-L-iduronic acid; 2-O-sulfo-L-iduronic acid; 2-O-sulfo-iduronic acid

N,O6-DISULFO-GLUCOSAMINE; 6-O-sulfo-N-sulfo-alpha-D-glucosamine; 2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucose; 2-deoxy-6-O-sulfo-2-(sulfoamino)-D-glucose; 2-deoxy-6-O-sulfo-2-(sulfoamino)-glucose

4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranuronic acid
4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enuronic acid; 4-deoxy-2-O-sulfo-L-threo-hex-4-enuronic acid; 4-deoxy-2-O-sulfo-threo-hex-4-enuronic acid

Structural and Functional Study of d-Glucuronyl C5-epimerase.

4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose

MRCLAAGVHYKTLIVICALLSLLTVLLWNKCTSEKALRFLPQHPQPPPSPKIDSHPQQPQPPEPPPVVGGVRYEEIDCLI
NDDATIKGRREGSEVYMPFSWMEKYFEVYGKVVQYDGYDRFEFSHSYSKVYAQREQYHPNGVFMSFEGYNVEVRDRVKCI
SGVEGVPLSTQWGPQGYFYAIQIAQYGLSHYSKNLTERPPHVEVYDTAEERDSRSSAWTVPKGCSLTRVYDKTRATSVRE
FSAPENSEGVSLPLGNTKDFIISFDLKFTSNGSVSVILETTEKGPPFVIHYVTTTQLILLKDRDITYGIGPRTTWTTVTR
DLLTDLRKGIGLSNTKAVKATKTMPRRVVKLVVHGTGTIDNITISTTSHMAAFYAASDWLVRNQDERGGWPIMVTRKLGE
GFRALEPGWYSAMAQGQAMSTLVRAYLMTKDDRYLKAALRATGPFKLPSEQHGVKAVFMNKYDWYEEYPTIPSSFVLNGF
IYSLIGLFDLAQTAGEKLGRDAGQLYSKGMESLKVMLPLYDTGSGTIYDLRHFILGTAPNLARWDYHTTHINQLQLLGTI
DNSPIFRDSVKRWKSYLKGGRAKHN

16% w/v PEG 3,350, 0.1 M sodium citrate tribasic dehydrate pH 5.6, and 2% v/v Tacsimate pH 5.0., VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystal structure of D-glucuronyl C5-epimerase

HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT

epimerization enzyme, multiple domain structure, Heparan sulfate C5-epimerase, heparin, heparan sulfate, ISOMERASE