Crystal Structure of the Computationally Designed Transmembrane Metallotransporter with 4-bromophenylalanine in Lipidic Cubic Phase
De novo design of a transmembrane Zn2+-transporting four-helix bundle.
Computationally Designed Transporter of Zn(II) and Proton
100 nl boluses containing monoolein-bound Rocker Br-Phe2 (4 mM peptide, 60% (vol) monoolein, 6 mM ZnSO4) immersed in 1 ?l of precipitation buffer (0.05 M Li2SO4, 0.1 M tricine (pH 7.4), 7 % (w/v) PEG 3000) incubated in 0.1-mm-deep wells at room temperature over a month
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
BR HAS ZERO OCCUPANCY IN 4BF. THE AUTHORS COLLECTED DATA FROM THE SINGLE CRYSTAL USING THE SYNCHROTRON BEAM TUNED AT THE BROMINE EDGE. BROMINE IS VERY LABILE UNDER RADIATION AND READILY DEGRADES UPON REPEATED DATA COLLECTION. THE LAST DATASET IS COLLECTED BY USING INCREASED EXPOSURE TO ATTAIN DIFFRACTIONS AT THE HIGH-RESOLUTION SPACE. THIS RESULTS IN OCCUPANCY OF 0 FOR THE MENTIONED BROMINE. THIS STUDY HAS BEEN CONFIRMED BY MASS SPEC
THE BIOLOGICAL UNIT WAS DETERMINED BY COMBINED APPROACH OF COMPUTATIONAL DESIGN, ANALYTICAL ULTRACENTRIFUGATION AND 19F-CODEX VIA SOLID-STATE NMR. AS PER THE AUTHORS THIS TETRAMERIC ASSEMBLY IS REPORTED IN THE SEPARATE ENTRY (PDB ID: 2MUZ) FOR THE SOLID-STATE NMR MODEL ACCOMPANIED BY THE PAPER THAT CITES THIS PDB
transmembrane, transporter, lipidic cubic phase, de-novo designed, DE NOVO PROTEIN