Satellite Tobacco Mosaic Virus Refined to 1.4 A Resolution using icosahedral constraints

Satellite tobacco mosaic virus refined to 1.4 angstrom resolution.

Refined structure of satellite tobacco mosaic virus at 1.8 A resolution.

Three-dimensional structure of satellite tobacco mosaic virus at 2.9 A resolution.

Double-helical RNA in satellite tobacco mosaic virus.

Macromolecular crystal growth experiments on International Microgravity Laboratory--1.

Preliminary analysis of crystals of satellite tobacco mosaic virus.

Satellite tobacco mosaic virus RNA: structure and implications for assembly.

A model for the structure of satellite tobacco mosaic virus.

arbitrary RNA adenine nucleotide decamer
RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3')

arbitrary RNA uridine nucleotide decamer
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3')

MGRGKVKPNRKSTGDNSNVVTMIRAGSYPKVNPTPTWVRAIPFEVSVQSGIAFKVPVGSLFSANFRTDSFTSVTVMSVRA
WTQLTPPVNEYSFVRLKPLFKTGDSTEEFEGRASNINTRASVGYRIPTNLRQNTVAADNVCEVRSNCRQVALVISCCFN

crystals grown from 15% ammonium sulfate on earth with a reservoir of 15% ammonium sulfate over 14 days., pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 296K

The same structure refined against room temperature data using strict icosahedral symmetry constraints

The same structure refined against room temperature data using NCS restraints

The same structure refined against 100 K temperature data using NCS restraints

icosahedral asymmetric unit, std point frame

Hydrogens were included in the refinements. Strict icosahedral symmetry was applied.

Double-helix RNA, Protein-RNA complex, Swiss Jelly-roll beta barrel, VIRUS-RNA complex