Crystal structure of SPF bound to squalene

Structural insights on cholesterol endosynthesis: Binding of squalene and 2,3-oxidosqualene to supernatant protein factor.

Alpha-tocopherol-associated protein, TAP, hTAP, Squalene transfer protein, Supernatant protein factor, SPF

GSHMSGRVGDLSPRQKEALAKFRENVQDVLPALPNPDDYFLLRWLRARSFDLQKSEAMLRKHVEFRKQKDIDNIISWQPP
EVIQQYLSGGMCGYDLDGCPVWYDIIGPLDAKGLLFSASKQDLLRTKMRECELLLQECAHQTTKLGRKVETITIIYDCEG
LGLKHLWKPAVEAYGEFLCMFEENYPETLKRLFVVKAPKLFPVAYNLIKPFLSEDTRKKIMVLGANWKEVLLKHISPDQV
PVEYGGTMTDPDGNPKCKSKINYGGDIPRKYYVRDQVK

0.2 M Lithium sulfate, 0.1 M Bis-Tris, 25% PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K

SUPERNATANT PROTEIN FACTOR IN COMPLEX WITH RRR-ALPHA-TOCOPHERYLQUINONE: A LINK BETWEEN OXIDIZED VITAMIN E AND CHOLESTEROL BIOSYNTHESIS

THE CRYSTAL STRUCTURE OF HUMAN SUPERNATANT PROTEIN FACTOR

Crystal structure of SPF bound to 2,3-oxidosqualene

cholesterol synthesis, squalene, 2, 3-oxidosqualene, SEC14-like, CRAL-TRIO domain, hydrophobic ligand transporter, TRANSPORT PROTEIN

MSGRVGDLSPRQKEALAKFRENVQDVLPALPNPDDYFLLRWLRARSFDLQKSEAMLRKHVEFRKQKDIDNIISWQPPEVI
QQYLSGGMCGYDLDGCPVWYDIIGPLDAKGLLFSASKQDLLRTKMRECELLLQECAHQTTKLGRKVETITIIYDCEGLGL
KHLWKPAVEAYGEFLCMFEENYPETLKRLFVVKAPKLFPVAYNLIKPFLSEDTRKKIMVLGANWKEVLLKHISPDQVPVE
YGGTMTDPDGNPKCKSKINYGGDIPRKYYVRDQVK