Ancestral Steroid Receptor 1 in complex with estrogen response element DNA

Evolution of DNA specificity in a transcription factor family produced a new gene regulatory module.

SKPKRLCQVCGDHASGFHYGVWSCEGCKAFFKRSIQGHVDYVCPATNNCTIDKHRRKSCQACRLRKCLEVGMTKGGQRKE
RR

12% PEG3350, 100 mM ammonium acetate, 100 mM Bis-Tris, pH 5.5, microseeded with a 1:100 dilution of crushed crystals of the same protein:DNA construct grown using higher concentration PEG3350 and 75 mM ammonium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K

Ancestral SR2 DBD helix mutant - ERE DNA complex

nuclear receptor zinc finger, transcription factor, steroid receptor DNA binding, estrogen receptor, TRANSCRIPTION-DNA complex