Neutralizing antibody to murine norovirus

Flexibility in surface-exposed loops in a virus capsid mediates escape from antibody neutralization.

QIVLTQSPAIMSASPGEKVTITCSASSSVSYMHWFQQKPGTSPKLWIYSTSNLASGVPARFSGSGSGTSYSLTISRMEAE
DAATYYCQQRSSYPFTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLN
SWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN

EVKLLESGGGLVQPGGSLKLSCAASGFDFSRYWMSWVRQAPGKGLEWIGQINPHSSTINYTPSLRDKFIISRDNAKNTLY
LQMTKVRSEDTALYYCARLLRYFYALDYWGQGASVTVSSAKTTPPSVYPLAPGRAAAAASMVTLGCLVKGYFPEPVTVTW
NSGSLAAGVHTFPAVLQAALYTLSSSVTVPSSSWPSETVTCNVAHPASSTKVDKKIVPRAA

Protein concentration 9.7 mg/ml in 50mM Tris. Reservoir 18% PEG 8000 in 90 mM Tris, pH 8.5. Drop was 5 microliters of reservoir and 5 of protein., VAPOR DIFFUSION, HANGING DROP, temperature 298K

There are two Fabs in the asymmetric unit. L (light) and H (heavy) chains make one and chains 1 and 2 make the other where 1=light and 2=heavy

Immunoglobin, antibody, murine norovirus, IMMUNE SYSTEM