Structure of Thermus thermophilus Argonaute bound to guide DNA and 19-mer target DNA with Mg2+

Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage.

MNHLGKTEVFLNRFALRPLNPEELRPWRLEVVLDPPPGREEVYPLLAQVARRAGGVTVRMGDGLASWSPPEVLVLEGTLA
RMGQTYAYRLYPKGRRPLDPKDPGERSVLSALARRLLQERLRRLEGVWVEGLAVYRREHARGPGWRVLGGAVLDLWVSDS
GAFLLEVDPAYRILCEMSLEAWLAQGHPLPKRVRNAYDRRTWELLRLGEEDPKELPLPGGLSLLDYHASKGRLQGREGGR
VAWVADPKDPRKPIPHLTGLLVPVLTLEDLHEEEGSLALSLPWEERRRRTREIASWIGRRLGLGTPEAVRAQAYRLSIPK
LMGRRAVSKPADALRVGFYRAQETALALLRLDGAQGWPEFLRRALLRAFGASGASLRLHTLHAHPSQGLAFREALRKAKE
EGVQAVLVLTPPMAWEDRNRLKALLLREGLPSQILNVPLREEERHRWENALLGLLAKAGLQVVALSGAYPAELAVGFDAG
GRESFRFGGAACAVGGDGGHLLWTLPEAQAGERIPQEVVWDLLEETLWAFRRKAGRLPSRVLLLRDGRVPQDEFALALEA
LAREGIAYDLVSVRKSGGGRVYPVQGRLADGLYVPLEDKTFLLLTVHRDFRGTPRPLKLVHEAGDTPLEALAHQIFHLTR
LYPASGFAFPRLPAPLHLADRLVKEVGRLGIRHLKEVDREKLFFV

(DT)(DG)(DA)(DG)(DG)(DT)(DA)(DG)(DT)(DA)(DG)(DG)(DT)(DT)(DG)(DT)(DA)(DT)(DA)(DG)
(DT)

18 mM magnesium chloride, 9% isopropanol, 50 mM sodium cacodylate, pH 6.5, 2.25 mM spermine, VAPOR DIFFUSION, HANGING DROP, temperature 306K

HALF (OCCUPANCY = 0.50) OF CHAIN D PRESENT IN THE CRYSTAL HAS BEEN CLEAVED TO FORM CHAIN P AND CONFORMATION B OF CHAIN D.

Argonaute, RNA interference, DNA interference, Piwi, NUCLEAR PROTEIN-DNA complex