X-Ray Crystal Structure of 2-amino-1-hydroxyethylphosphonate-bound PhnZ

[(1R)-2-amino-1-hydroxyethyl]phosphonic acid

Organophosphonate-degrading PhnZ reveals an emerging family of HD domain mixed-valent diiron oxygenases.

MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQH
EKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAW
DEKGKQTDLKVPGPEHYRKMMEEHLSENQNLEHHHHHH

0.1 M sodium acetate, 0.2 M ammonium sulphate, 25%(w/v)PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 294K

MSLSNSSKVSVLISLLEKSRDLDYIGEAINQLEHSLQCAYFAQRSGADNEMVLAALLHDLGHYCNDTSFEDMGGYGVWQH
EKVGADYLRGLGFSERVACLIEGHVAAKRYLVSSKASYLKNLSDASRKTLEYQGGPMDEGERRLFEEREDFKDCLKIRAW
DEKGKQTDLKVPGPEHYRKMMEEHLSENQN