Kuenenia stuttgartiensis hydroxylamine oxidoreductase soaked in hydrazine

Structural Basis of Biological NO Generation by Octaheme Oxidoreductases.

Similar to hydroxylamine oxidoreductase hao

EGPTFQDVASQVFGQPVGPDNDGTLYIFGLTAKYTEPEYVDGRGPYKSFLKMLPSIRWYDPEHYWTNGSQTEGVFKNEEC
VLCHTVQTPTIVNDWKQSSHGSKDIRRGIGIKKDGKPVEDLVGCADCHGNNHQKLEMPTYKLCNDCHPKETAEHRAGGLG
SHTHAYTVNVLEFSWHVGKPAEEVTGCAHCHAIAENRCSGCHTRHKFDPAEARKPTACRVCHMGIDHDEWAMYNTSIHGA
LYEAESARMDWGKKLKKGNYRVPTCAYCHMQNGDHNPQRFGTIYSDMGMFQVDRGAPKHKAKRDSWIKLCQDCHSPRFAA
DKLKEMDAGVNLSFTKWREAAAVIVGCYLDGVVDPMPEGSAPDWYGHYTFSLLPGGDPRFYATSNLERLGLEMICYLTGN
VYKAYAHMSMYNQTYGNGSAFEQDRKLVEIKTEAAKLRRFAAIEKKIGLEHKSADFWKHGEYLDLLPGWKRKPGDVDVEW
FKRTDIPHRANADAGVEIHH

1.3 M ammonium sulfate, 0.05-0.10 M sodium phosphate, pH 7.4, detergent additive cyclohexylbutanoyl-N-hydroxyethylglucamide (C-HEGA-10), VAPOR DIFFUSION, HANGING DROP, temperature 293K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Covalent trimer confirmed by SDS-PAGE, analytical ultracentrifugation, and gel filtration