Crystal Structure of the alpha-L-arabinofuranosidase PaAbf62A from Podospora anserina in complex with cellotriose

4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID

First Structural Insights into alpha-L-Arabinofuranosidases from the Two GH62 Glycoside Hydrolase Subfamilies.

SSLPAGRDVSIVQLSNQPPSDLPTTWQWTSTGPLVGPKNDGRGIAGIKDPTIILINGTHHVFASTAQSAGYNLVYFTFAD
WADAPNATFYYLDQAPLGTGYRAAPQVFWFAPHKLWYLVYQNGNAAYSTNPDINNPKGWTAPKVFYPDGMPKIIEQNIGE
GYWVDMWVICDSASCHLFSSDDNGQLYRSETSLEQFPNGMSQPVIAMQDNRNDLFEAACVYSLPDGKYLLLVEAIGTDGH
RWFRSWTADSIRGPWQGLANTEQNPWARSNNVQFDGDVWTKSISHGEIIRDGTVDEKLLIDPCNIRFMYQGMDPSAGGEY
NALPWRLGFIAHNNPACGLEQKLISEEDLNSAVDHHHHHH

25% PEG 4000, 0.2M Calcium chloride, 0.1M Tris , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K

The same protein in complex with L-arabinofuranose

alpha-L-arabinofuranosidase PaAbf62A from Podospora anserina

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Beta-Propeller, Hydrolase, hemicellulose binding

SSLPAGRDVSIVQLSNQPPSDLPTTWQWTSTGPLVGPKNDGRGIAGIKDPTIILINGTHHVFASTAQSAGYNLVYFTFAD
WADAPNATFYYLDQAPLGTGYRAAPQVFWFAPHKLWYLVYQNGNAAYSTNPDINNPKGWTAPKVFYPDGMPKIIEQNIGE
GYWVDMWVICDSASCHLFSSDDNGQLYRSETSLEQFPNGMSQPVIAMQDNRNDLFEAACVYSLPDGKYLLLVEAIGTDGH
RWFRSWTADSIRGPWQGLANTEQNPWARSNNVQFDGDVWTKSISHGEIIRDGTVDEKLLIDPCNIRFMYQGMDPSAGGEY
NALPWRLGFIAHNNPAC