Complex structure of Arterivirus nonstructural protein 10 (helicase) with DNA

Structural basis for the regulatory function of a complex zinc-binding domain in a replicative arterivirus helicase resembling a nonsense-mediated mRNA decay helicase.

ORF1ab polyprotein, Nsp1 papain-like cysteine proteinase, PCP, Nsp2 cysteine proteinase, CP2, CP, Non-structural protein 3, Nsp3, 3C-like serine proteinase, 3CLSP, Nsp4, Non-structural protein 5-6-7, Nsp5-6-7, Non-structural protein 5, Nsp5, Non-structural protein 6, Nsp6, Non-structural protein 7-alpha, Nsp7-alpha, Non-structural protein 7-beta, Nsp7-beta, Non-structural protein 8, Nsp8, RNA-directed RNA polymerase, Pol, RdRp, Nsp9, Helicase, Hel, Nsp10, Non-structural protein 11, Nsp11, Non-structural protein 12, Nsp12

MGSSHHHHHSSGENLYFQGHMSAVCTVCGAAPVAKSACGGWFCGNCVPYHAGHCHTTSLFANCGHDIMYRSTYCTMCEGS
PKQMVPKVPHPILDHLLCHIDYGSKEELTLVVADGRTTSPPGRYKVGHKVVAVVADVGGNIVFGCGPGSHIAVPLQDTLK
GVVVNKALKNAAASEYVEGPPGSGKTFHLVKDVLAVVGSATLVVPTHASMLDCINKLKQAGADPYFVVPKYTVLDFPRPG
SGNITVRLPQVGTSEGETFVDEVAYFSPVDLARILTQGRVKGYGDLNQLGCVGPASVPRNLWLRHFVSLEPLRVCHRFGA
AVCDLIKGIYPYYEPAPHTTKVVFVPNPDFEKGVVITAYHKDRGLGHRTIDSIQGCTFPVVTLRLPTPQSLTRPRAVVAV
TRASQELYIYDPFDQLSGLLKFT

1.2M NH4SO4, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K

HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT

arterivirus, helicase, zbd, nsp10, complex, HYDROLASE-DNA complex

SAVCTVCGAAPVAKSACGGWFCGNCVPYHAGHCHTTSLFANCGHDIMYRSTYCTMCEGSPKQMVPKVPHPILDHLLCHID
YGSKEELTLVVADGRTTSPPGRYKVGHKVVAVVADVGGNIVFGCGPGSHIAVPLQDTLKGVVVNKALKNAAASEYVEGPP
GSGKTFHLVKDVLAVVGSATLVVPTHASMLDCINKLKQAGADPYFVVPKYTVLDFPRPGSGNITVRLPQVGTSEGETFVD
EVAYFSPVDLARILTQGRVKGYGDLNQLGCVGPASVPRNLWLRHFVSLEPLRVCHRFGAAVCDLIKGIYPYYEPAPHTTK
VVFVPNPDFEKGVVITAYHKDRGLGHRTIDSIQGCTFPVVTLRLPTPQSLTRPRAVVAVTRASQELYIYDPFDQLSGLLK
FT