Conserved Residues that Modulate Protein trans-Splicing of Npu DnaE Split Intein
Nucleic acid binding, OB-fold, tRNA/helicase-type
HHHHHHCLSYETEILTVEYGLLPIGKIVEKRIECTVYSVDNNGNIYTQPVAQWHDRGEQEVFEYCLEDGSLIRATKDHKF (MSE)TVDGQ(MSE)LPIDEIFERELDL(MSE)RVDNLPN
HHHHHHCLSYETEILTVEYGLLPIGKIVEKRIECTVYSVDNNGNIYTQPVAQWHDRGEQEVFEYCLEDGSLIRATKDHKF MTVDGQMLPIDEIFERELDLMRVDNLPN
20mM Tris-HCl, 100mM NaCl, 2mM Dithiothreitol, pH 8.0, VAPOR DIFFUSION, SITTING DROP
trans-structure, naturally occurring, TRANSFERASE
CLSYETEILTVEYGLLPIGKIVEKRIECTVYSVDNNGNIYTQPVAQWHDRGEQEVFEYCLEDGSLIRATKDHKFMTVDGQ MLPIDEIFERELDLMRVDNLPN