Structure of an active ligase (HOIP-H889A)/ubiquitin transfer complex

Structural basis for ligase-specific conjugation of linear ubiquitin chains by HOIP.

E3 ligase HOIP catalytic core (unp residues 853-1072)

HOIL-1-interacting protein, HOIP, RING finger protein 31, Zinc in-between-RING-finger ubiquitin-associated domain protein

GPGPEYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFACTQCRHQFCSGCYNAFYAKNKCPEPNCRVKKSLHGHHPRD
CLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSL
INAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRRK

0.1 M amino acids, 0.1 M imidazole, MES, 30 % P550 MME_P20K , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

chain 'A' and (resid 1013 through 1060 )

chain 'A' and (resid 1061 through 1071 )

E3 ligase-ubiquitin complex, LIGASE, HOIP, RNF31, ubiquitin, RBR ligase, E3 ligase, RING domain, IBR domain, Zinc Finger

PEYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPEPNCRVKKSLHGHHPRDCLF
YLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINA
HSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRRK