Cyclic GMP-AMP Synthase Is Activated by Double-Stranded DNA-Induced Oligomerization.

human cGAS catalytic domain, UNP residues 157-522

cGAMP synthase, cGAS, h-cGAS, Mab-21 domain-containing protein 1

FELDAAPGASKLRAVLEKLKLSRDDISTAAG(MSE)VKGVVDHLLLRLKCDSAFRGVGLLNTGSYYEHVKISAPNEFDV
(MSE)FKLEVPRIQLEEYSNTRAYYFVKFKRNPKENPLSQFLEGEILSASK(MSE)LSKFRKIIKEEINDIKDTDVI
(MSE)KRKRGGSPAVTLLISEKISVDITLALESKSSWPASTQEGLRIQNWLSAKVRKQLRLKPFYLVPKHAKEGNGFQEE
TWRLSFSHIEKEILNNHGKSKTCCENKEEKCCRKDCLKL(MSE)KYLLEQLKERFKDKKHLDKFSSYHVKTAFFHVCTQN
PQDSQWDRKDLGLCFDNCVTYFLQCLRTEKLENYFIPEFNLFSSNLIDKRSKEFLTKQIEYERNNEFPVFDEF

FELDAAPGASKLRAVLEKLKLSRDDISTAAGMVKGVVDHLLLRLKCDSAFRGVGLLNTGSYYEHVKISAPNEFDVMFKLE
VPRIQLEEYSNTRAYYFVKFKRNPKENPLSQFLEGEILSASKMLSKFRKIIKEEINDIKDTDVIMKRKRGGSPAVTLLIS
EKISVDITLALESKSSWPASTQEGLRIQNWLSAKVRKQLRLKPFYLVPKHAKEGNGFQEETWRLSFSHIEKEILNNHGKS
KTCCENKEEKCCRKDCLKLMKYLLEQLKERFKDKKHLDKFSSYHVKTAFFHVCTQNPQDSQWDRKDLGLCFDNCVTYFLQ
CLRTEKLENYFIPEFNLFSSNLIDKRSKEFLTKQIEYERNNEFPVFDEF

20-30% Jeffamine M-600, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

DAAPGASKLRAVLEKLKLSRDDISTAAGMVKGVVDHLLLRLKCDSAFRGVGLLNTGSYYEHVKISAPNEFDVMFKLEVPR
IQLEEYSNTRAYYFVKFKRNPKENPLSQFLEGEILSASKMLSKFRKIIKEEINDIKDTDVIMKRKRGGSPAVTLLISEKI
SVDITLALESKSSWPASTQEGLRIQNWLSAKVRKQLRLKPFYLVPKHAKEGNGFQEETWRLSFSHIEKEILNNHGKSKTC
CENKEEKCCRKDCLKLMKYLLEQLKERFKDKKHLDKFSSYHVKTAFFHVCTQNPQDSQWDRKDLGLCFDNCVTYFLQCLR
TEKLENYFIPEFNLFSSNLIDKRSKEFLTKQIEYERNNEFPVFDEF