Crystal structure of near-infrared fluorescent protein with an extended stokes shift, pH 4.5

New York Structural Genomics Research Consortium (NYSGRC)

{(4Z)-4-(4-hydroxybenzylidene)-2-[3-(methylthio)propanimidoyl]-5-oxo-4,5-dihydro-1H-imidazol-1-yl}acetic acid

Extended Stokes shift in fluorescent proteins: chromophore-protein interactions in a near-infrared TagRFP675 variant.

MGSHHHHHHGRSMSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTQRIKVVEGGPLPFAFDILATSF(NRQ)S
KTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEM
MYPADGGLEGRNYMALKLVGGGHLICSLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYCDLPS
KLGHKLN

MGSHHHHHHGRSMSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTQRIKVVEGGPLPFAFDILATSFMYGSKT
FINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMMY
PADGGLEGRNYMALKLVGGGHLICSLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYCDLPSKL
GHKLN

0.1M Na-acetate, pH 4.5, 3.0M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K

New York Structural Genomics Research Consortium

U VALUES : WITH TLS ADDED HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT

STRUCTURAL GENOMICS, PROTEINSTRUCTURE INITIATIVE, NEW YORK STRUCTURAL GENOMIX RESEARCHCONSORTIUM, HYDROLASE, PSI-Biology, New York Structural Genomics Research Consortium, NYSGRC, FLUORESCENT PROTEIN