Crystal structure of PUD-1/PUD-2 heterodimer

Characterization of PUD-1 and PUD-2, two proteins up-regulated in a long-lived daf-2 mutant.

SQLTRRTAKVSIDNQTGSHFKFQVTHKYTGWDADKSDVVMFQPDEVKEIFKSVAYNTGFLTTGVDNWLVDGTMVQERTEV
DNKGHQIGKKSYIEHAKFISDSRSWKQHMLTAEDDGKTTTIRVFPTEIHFISPSGESTTTFTKY

SGVTEQWAKVDIENKSDHVFKFQVLHQYTGNALEASKWVKLEPNQSAQILEKVHYNTGPFTTGTDNWKVHGIKQIETNLD
DKNGVVDGKVRILGEAWRSGHPDGADWKKHTLRVEDHAQTTVIKVLEKEVQFVSKSGTSTTDFYRH

0.1M HEPES (pH 7.2), 4.3M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K

HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT U VALUES: REFINED INDIVIDUALLY

QLTRRTAKVSIDNQTGSHFKFQVTHKYTGWDADKSDVVMFQPDEVKEIFKSVAYNTGFLTTGVDNWLVDGTMVQERTEVD
NKGHQIGKKSYIEHAKFISDSRSWKQHMLTAEDDGKTTTIRVFPTEIHFISPSGESTTTFTKY