Catalytic amidase domain of the major autolysin LytA from Streptococcus pneumaniae

Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae.

Cell wall hydrolase, Mucopeptide aminohydrolase, Murein hydrolase, N-acetylmuramoyl-L-alanine amidase

ASMEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWRKDPELGFFSHIVGNGAIMQVGPVDNGAWDVGGGW
NAETYAAVELIESHSTKEEFMTDYRLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYATNNQPNNHSDHVDPYPYLAKWG
ISREQFKHDIENGLTIETGWQKHHHHHH

PEG 6000 12%, 0.1M TrisHCl, LiCl 0.8M, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

amidase-2, LytA, peptidoglycan cleavage, autolysin, HYDROLASE

MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWRKDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNA
ETYAAVELIESHSTKEEFMTDYRLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
REQFKHDIENGLTIETGWQKN