Crystal structure of chemically synthesized human anaphylatoxin C3a
Efficient chemical synthesis of human complement protein C3a.
C3a anaphylatoxin (UNP residues 672-748)
VAPOR DIFFUSION, HANGING DROP, 1 uL 5 mg/mL lyophilized C3a in 2 mM hydrochloric acid + 1 uL reservoir (0.1 M Tris-HCl, 0.2 M ammonium phosphate monobasic, 49-51% v/v MPD, pH 8.5) against 500 uL reservoir, prepared at 277K, incubated at 281K for 96 hours, stored at 277K, rhomboid-shaped crystals appeared after 6 days and grew to a final size of 0.5 x 0.2 x 0.2 mm
native chemical ligation of three polypeptide segments
HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
disulfide-rich, alpha-helical bundle, drumstick, complement system, third component of complement (C3), local inflammatory process mediator, smooth muscle contraction induction, vascular permeability increase, mast cell histamine release, C3a receptor, GPCR, extracellular, blood plasma, IMMUNE SYSTEM