Crystal structure of yeast Ap4A phosphorylase Apa2 in complex with Ap4A

Structures of yeast Apa2 reveal catalytic insights into a canonical AP4A phosphorylase of the histidine triad superfamily

5',5'''-P-1,P-4-tetraphosphate phosphorylase 2

ATP adenylyltransferase, Ap4A phosphorylase II, AP,A phosphorylase, Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase, AP-4-A phosphorylase

MGHHHHHHMIEENLKQKIHDKFVAAKKNGHLKVTHAESKKLKDPQTTTQYWVTFAPSLALKPDANKNSDSKAEDPFANPD
EELVVTEDLNGDGEYKLLLNKFPVVPEHSLLVTSEFKDQRSALTPSDLMTAYNVLCSLQGDKDDDVTCERYLVFYNCGPH
SGSSQDHKALQIMQMPEKFIPFQDVLCNGKDHFLPTFNAEPLQDDKVSFAHFVLPLPESSDQVDEDLLAMCYVSLMQRAL
TFFQDWTNESPELTKSYNVLLTKKWICVVPRSHAKSGPPLMLNINSTGYCGMILVKDREKLENLTEDPHLVDKSLLQCGF
PNTAGQKPTEYHY

23% PEG 2K, 0.1M Tris-HCl, pH 8.0, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 289K

5',5'''-P-1,P-4-tetraphosphate phosphorylase 2 (E.C.2.7.7.53)

alpha/beta fold, Ap4A phosphorylase, Ap4A, TRANSFERASE

MIEENLKQKIHDKFVAAKKNGHLKVTHAESKKLKDPQTTTQYWVTFAPSLALKPDANKNSDSKAEDPFANPDEELVVTED
LNGDGEYKLLLNKFPVVPEHSLLVTSEFKDQRSALTPSDLMTAYNVLCSLQGDKDDDVTCERYLVFYNCGPHSGSSQDHK
HLQIMQMPEKFIPFQDVLCNGKDHFLPTFNAEPLQDDKVSFAHFVLPLPESSDQVDEDLLAMCYVSLMQRALTFFQDWTN
ESPELTKSYNVLLTKKWICVVPRSHAKSGPPLMLNINSTGYCGMILVKDREKLENLTEDPHLVDKSLLQCGFPNTAGQKP
TEYHY