A thermostable variant of human NUDT18 NUDIX domain obtained by Hot Colony Filtration

Engineering protein thermostability using a generic activity-independent biophysical screen inside the cell.

Nucleoside diphosphate-linked moiety X motif 18
NUDIX hydrolase domain containing residues 26-179

MSAPAGEPPAPVRLRKNVCYMVLAVFLSEQDEVLLIQEAKRECRGSWYLPAGRMEPGETIVEALQRVVKEEAGLHCEPET
LLSVEERGPSWVRFVFLARPTGGILKTSKEADAESLQAAWYPRTSLPTPLRAHDILHLVELAAQYRQQARHPLILAHHHH
HH

0.1M tri-sodium citrate, 3.2M ammonium sulfate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K

hydrolase, thermostability, directed evolution, Hot-CoFi, Hot Colony Filtration

SAPAGEPPAPVRLRKNVCYVVLAVFLSEQDEVLLIQEAKRECRGSWYLPAGRMEPGETIVEALQREVKEEAGLHCEPETL
LSVEERGPSWVRFVFLARPTGGILKTSKEADAESLQAAWYPRTSLPTPLRAHDILHLVELAAQYRQQARHPLIL