Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGTTACGGT)
Nonspecific Recognition Is Achieved in Pot1pC through the Use of Multiple Binding Modes.
Pot1pC, partial DNA binding domain, residues 198-339
DNA (5'-D(*GP*GP*TP*TP*AP*CP*GP*GP*T)-3')
(MSE)SDSFSLLSQITPHQRCSFYAQVIKTWYSDKNFTLYVTDYTENELFFP(MSE)SPYTSSSRWRGPFGRFSIRCILW DEHDFYCRNYIKEGDYVV(MSE)KNVRTKIDHLGYLECILHGDSAKRYN(MSE)SIEKVDSEEPELNEIKSRKRLYVQN
MSDSFSLLSQITPHQRCSFYAQVIKTWYSDKNFTLYVTDYTENELFFPMSPYTSSSRWRGPFGRFSIRCILWDEHDFYCR NYIKEGDYVVMKNVRTKIDHLGYLECILHGDSAKRYNMSIEKVDSEEPELNEIKSRKRLYVQN
25% w/V PEG 4000, 0.2M ammonium formate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 290K
The same protein bound to a different ssDNA sequence
Protection of telomeres protein 1/DNA complex
specificity, plasticity, promiscuity, OB-fold, ssDNA binding, single-stranded telomeric DNA, DNA BINDING PROTEIN
SVSFSLLSQITPHQRCSFYAQVIKTWYSDKNFTLYVTDYTENELFFPMSPYTSSSRWRGPFGRFSIRCILWDEHDFYCRN YIKEGDYVVMKNVRTKIDHLGYLECILHGDSAKRYNMSIEKVDSEEPELNEIKSRKRLYVQN