Crystal Structure of BamB from Pseudomonas aeruginosa

Crystal structure of BamB from Pseudomonas aeruginosa and functional evaluation of its conserved structural features.

Outer membrane protein assembly factor BamB

GAMASSNSKKELPPAELTDFKEEVVLSKQWSRSVGDGQGDLYNLLEPAVDGSTIYAASAEGRVMAIQRETGDVLWKKDLE
RPVSGGVGVGYGLVLVGTLRGDVIALDEATGKKKWTKRVNSEVLSAPATNGDVVVVQTQDDKLIGLDAASGDQRWIYEST
VPVLTLRGTGAPLIAGNMALAGLASGKVVAVDVQRGLPIWEQRVAIPQGRSELDRVVDIDGGLLLSGDTLYVVSYQGRAA
ALDVNSGRLLWQREASSYVGVAEGFGNIYVSQASGSVEGLDSRGASSLWNNDALARRQLSAPAVFSSNVVVGDLEGYVHL
LSQVDGRFVGRERVDSDGVRVRPLVVGSWMYVFGNGGKLVAYTIRPG

10% Tacsimate, 10% PEG , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289.15K

beta-propeller, beta-barrel assembly, PROTEIN BINDING

SSNSKKELPPAELTDFKEEVVLSKQWSRSVGDGQGDLYNLLEPAVDGSTIYAASAEGRVMAIQRETGDVLWKKDLERPVS
GGVGVGYGLVLVGTLRGDVIALDEATGKKKWTKRVNSEVLSAPATNGDVVVVQTQDDKLIGLDAASGDQRWIYESTVPVL
TLRGTGAPLIAGNMALAGLASGKVVAVDVQRGLPIWEQRVAIPQGRSELDRVVDIDGGLLLSGDTLYVVSYQGRAAALDV
NSGRLLWQREASSYVGVAEGFGNIYVSQASGSVEGLDSRGASSLWNNDALARRQLSAPAVFSSNVVVGDLEGYVHLLSQV
DGRFVGRERVDSDGVRVRPLVVGSWMYVFGNGGKLVAYTIR