Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex

Crystal structure of pyridoxine 4-oxidase from Mesorhizobium loti.

NCDIVIVGGGSAGSLLAARLSEDPDSRVLLIEAGEEPTDPDIWNPAAWPALQGRSYDWDYRTEAQAGTAGRAHHWARGRL
IGGSS(CME)LHAMGYMRGHPSDFQAWVDASGDRRWGWDELLPVFQAIEDHPLGGDGIHGKGGPLPIHLPADEVSPLARA
FIEAGASLGLPRLEGHNSGEMIGVTPNSLNIRDGRRVTAADAWLTKAVRGRKNLTILTGSRVRRLKLEGNQVRSLEVVGR
QGSAEVFADQIVLCAGALESPALLMRSGIGPHDVLDAAGVG(CME)LIDMPDIGRNLQDHLLGAGNLYAARKPVPPSRLQ
HSESMAYMRADSFTAAGQPEIVVGCGVAPIVSESFPAPAAGSAYSLLFGITHPTSRGSVRISGPELGDRLIIDPAYLQTG
RDRERFRRALEASRTIGHRDELAGWRERELLPGTPNSAAEMDDFIARSVITHHHPCGTCRMGKDPDAVVDANLRLKALDN
LFVVDASIMPNLTAGPIHAAVLAIAETFARQYHHHH

NCDIVIVGGGSAGSLLAARLSEDPDSRVLLIEAGEEPTDPDIWNPAAWPALQGRSYDWDYRTEAQAGTAGRAHHWARGRL
IGGSSCLHAMGYMRGHPSDFQAWVDASGDRRWGWDELLPVFQAIEDHPLGGDGIHGKGGPLPIHLPADEVSPLARAFIEA
GASLGLPRLEGHNSGEMIGVTPNSLNIRDGRRVTAADAWLTKAVRGRKNLTILTGSRVRRLKLEGNQVRSLEVVGRQGSA
EVFADQIVLCAGALESPALLMRSGIGPHDVLDAAGVGCLIDMPDIGRNLQDHLLGAGNLYAARKPVPPSRLQHSESMAYM
RADSFTAAGQPEIVVGCGVAPIVSESFPAPAAGSAYSLLFGITHPTSRGSVRISGPELGDRLIIDPAYLQTGRDRERFRR
ALEASRTIGHRDELAGWRERELLPGTPNSAAEMDDFIARSVITHHHPCGTCRMGKDPDAVVDANLRLKALDNLFVVDASI
MPNLTAGPIHAAVLAIAETFARQYHHHH

Hepes Na-salt(pH 7.5) 0.1M, 2-propanol 10%,PEG 4000 24%, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Substrate binding domain, FAD binding domain, beta alpha beta fold, substrate access tunnel, ADP binding, OXIDOREDUCTASE

NCDIVIVGGGSAGSLLAARLSEDPDSRVLLIEAGEEPTDPDIWNPAAWPALQGRSYDWDYRTEAQAGTAGRAHHWARGRL
IGGSSCLHAMGYMRGHPSDFQAWVDASGDRRWGWDELLPVFQAIEDHPLGGDGIHGKGGPLPIHLPADEVSPLARAFIEA
GASLGLPRLEGHNSGEMIGVTPNSLNIRDGRRVTAADAWLTKAVRGRKNLTILTGSRVRRLKLEGNQVRSLEVVGRQGSA
EVFADQIVLCAGALESPALLMRSGIGPHDVLDAAGVGCLIDMPDIGRNLQDHLLGAGNLYAARKPVPPSRLQHSESMAYM
RADSFTAAGQPEIVVGCGVAPIVSESFPAPAAGSAYSLLFGITHPTSRGSVRISGPELGDRLIIDPAYLQTGRDRERFRR
ALEASRTIGHRDELAGWRERELLPGTPNSAAEMDDFIARSVITHHHPCGTCRMGKDPDAVVDANLRLKALDNLFVVDASI
MPNLTAGPIHAAVLAIAETFARQY