Role of the biradical intermediate observed during the turnover of SLAC: A two-domain laccase from Streptomyces coelicolor

Involvement of Tyr108 in the enzyme mechanism of the small laccase from Streptomyces coelicolor

TARTAPAGGEVRHLKMYAEKLADGQMGYGFEKGKASVPGPLIEVNEGDTLHIEFTNTMDVRASLHVHGLDFEISSDGTAM
NKSDVEPGGTRTYTWRTHKPGRRDDGTWRPGSAGYWHYHDHVVGTEHGTGGIRNGLYGPVIVRRKGDVLPDATHTIVFND
MTINNRKPHTGPDFEATVGDRVEIVMITHGEYYHTFHMHGHRWADNRTGILTGPDDPSRVIDNKITGPADSFGFQIIAGE
GVGAGAWMYHCHVQSHSDMGMVGLFLVKKPDGTIPGYEP

35-45% polyethylene glycol-550-monomethyl ether, 0.15-0.5M NaCl, 0.1M TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K

HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT

TARTAPAGGEVRHLKMYAEKLADGQMGYGFEKGKASVPGPLIEVNEGDTLHIEFTNTMDVRASLHVHGLDYEISSDGTAM
NKSDVEPGGTRTYTWRTHKPGRRDDGTWRPGSAGYWHYHDHVVGTEHGTGGIRNGLYGPVIVRRKGDVLPDATHTIVFND
MTINNRKPHTGPDFEATVGDRVEIVMITHGEYYHTFHMHGHRWADNRTGILTGPDDPSRVIDNKITGPADSFGFQIIAGE
GVGAGAWMYHCHVQSHSDMGMVGLFLVKKPDGTIPGYEP