His 62 mutant of the lectin binding domain of lectinolysin complexed with Lewis y

alpha-L-fucose; 6-deoxy-alpha-L-galactopyranose; L-fucose; fucose

beta-D-galactose; D-galactose; galactose

N-acetyl-beta-D-glucosamine; 2-acetamido-2-deoxy-beta-D-glucose; 2-acetamido-2-deoxy-D-glucose; 2-acetamido-2-deoxy-glucose; N-ACETYL-D-GLUCOSAMINE

Manipulating the Lewis antigen specificity of the cholesterol-dependent cytolysin lectinolysin

lectin binding domain, UNP residues 38-190

EQGNRPVETENIARGKQASQSSTAHGGAATRAVDGNVDSDYGHHSVTHTNFEDNAWWQVDLGKTENVGKVKLYNRGDGNV
ANRLSNFDVVLLNEAKQEVARQHFDSLNGKAELEVFFTAKDARYVKVELKTKNTPLSLAEVEVFRSATTQVGC

2.4M MgSO4, 0.1M KCl, 0.1M Tris, pH 8.75, VAPOR DIFFUSION, HANGING DROP, temperature 294K

lectin binding domain complexed with Lewis B

lectin binding domain complexed with Lewis Y

lectin binding domain complexed with fucose

lectin binding domain complexed with glycerol

THE SEQUENCE WAS DERIVED FROM THE PUBLISHED PROTEIN SEQUENCE PUBLISHED BY FARRAND ET AL. (BIOCHEMISTRY, VOL. 47, 2008). AMINO ACIDS 67 AND 158 ARE THREONINE AND ASPARTATE, RESPECTIVELY.

HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT

cholesterol-dependent cytolysins, lewis antigens, F-type lectin fold, glycan binding, SUGAR BINDING PROTEIN

EQGNRPVETENIARGKQASQSSTAYGGAAARAVDGNVDSDYGHHSVTHTNFEDNAWWQVDLGKTENVGKVKLYNRGDGNV
ANRLSNFDVVLLNEAKQEVARQHFDSLNGKAELEVFFTAKAARYVKVELKTKNTPLSLAEVEVFRSATTQVGQ