Crystal Structure of a GH7 Family Cellobiohydrolase from Limnoria quadripunctata

Structural characterization of a unique marine animal family 7 cellobiohydrolase suggests a mechanism of cellulase salt tolerance

Molecular insight into lignocellulose digestion by a marine isopod in the absence of gut microbes.

(PCA)QAGTETEEYHLPLTWERDGSSVSASVVIDSNWRWTHSTEDTTNCYDGNEWDSTLCPDADTCTENCAIDGVDQGTW
GDTYGITASGSKLTLSFVTEGEYSTDIGSRVFLMADDDNYEIFNLLDKEFSFDVDASNLPCGLNGALYFVSMDEDGGTSK
YSTNTAGAKYGTGYCDAQCPHDMKFIAGKANSDGWTPSDNDQNAGTGEMGACCHEMDIWEANSQAQSYTAHVCSVDGYTP
CTGTDCGDNGDDRYKGVCDKDGCDYAAYRLGQHDFYGEGGTVDSGSTLTVITQFITGGGGLNEIRRIYQQGGQTIQNAAV
NFPGDVDPYDSITEDFCVDIKRYFGDTNDFDAKGGMSGMSNALKKGMVLVMSLWDDHYANMLWLDATYPVDSTEPGALRG
PCSTDSGDPADVEANFPGSTVTFSNIKIGPIQSYD

QQAGTETEEYHLPLTWERDGSSVSASVVIDSNWRWTHSTEDTTNCYDGNEWDSTLCPDADTCTENCAIDGVDQGTWGDTY
GITASGSKLTLSFVTEGEYSTDIGSRVFLMADDDNYEIFNLLDKEFSFDVDASNLPCGLNGALYFVSMDEDGGTSKYSTN
TAGAKYGTGYCDAQCPHDMKFIAGKANSDGWTPSDNDQNAGTGEMGACCHEMDIWEANSQAQSYTAHVCSVDGYTPCTGT
DCGDNGDDRYKGVCDKDGCDYAAYRLGQHDFYGEGGTVDSGSTLTVITQFITGGGGLNEIRRIYQQGGQTIQNAAVNFPG
DVDPYDSITEDFCVDIKRYFGDTNDFDAKGGMSGMSNALKKGMVLVMSLWDDHYANMLWLDATYPVDSTEPGALRGPCST
DSGDPADVEANFPGSTVTFSNIKIGPIQSYD

0.1M Bis-Tris, 25% PEG 3350, 0.2M MgCl2, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K

HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT U VALUES: REFINED INDIVIDUALLY

cellobiohydrolase, Glycosyl hydrolase, hydrolase

QAGTETEEYHLPLTWERDGSSVSASVVIDSNWRWTHSTEDTTNCYDGNEWDSTLCPDADTCTENCAIDGVDQGTWGDTYG
ITASGSKLTLSFVTEGEYSTDIGSRVFLMADDDNYEIFNLLDKEFSFDVDASNLPCGLNGALYFVSMDEDGGTSKYSTNT
AGAKYGTGYCDAQCPHDMKFIAGKANSDGWTPSDNDQNAGTGEMGACCHEMDIWEANSQAQSYTAHVCSVDGYTPCTGTD
CGDNGDDRYKGVCDKDGCDYAAYRLGQHDFYGEGGTVDSGSTLTVITQFITGGGGLNEIRRIYQQGGQTIQNAAVNFPGD
VDPYDSITEDFCVDIKRYFGDTNDFDAKGGMSGMSNALKKGMVLVMSLWDDHYANMLWLDATYPVDSTEPGALRGPCSTD
SGDPADVEANFPGSTVTFSNIKIGPIQSYD