Crystal Structure of HmtT Involved in Himastatin Biosynthesis

Structural analysis of HmtT and HmtN involved in the tailoring steps of himastatin biosynthesis

MGSSHHHHHHSSGLVPRGSHMMTTVVDRWNIHPDHLWLRGQRPESPVVFDETQGVWNVYGYPEAMDILNDHDTFTSDLAH
LLPVSVDAPLLEGDMSQMDPPRHRKYRQLVSRAFTPRLVADMETRVADITRELLDAVDGKPEIEIAADLAYPLPVIVIAE
LLGVPAGDRDLFKKWADDIIEGFSGFSFLDTSGQGEQDVRDATERLRPLLDYMAGHVTERRRTPREDLLTHLVQAEVDGE
RLTDNEIVNVANILLVTGHITTTMTLGNTVLCLDADPEVAAKVRADRSLVPGAIEEALRVLSPSAALARGTSREVEVAGT
VIPKDQIVMLWLGAGNRDPRQFTDPEVYDPTRDPNPHFGFGRGIHFCLGAPLARLEGRVALNALFDRFPVLRTDPAKPPT
FFPTPDMIGVNTLHLRTS

8%(w/v) PEG4000, 3%(v/v) Dimethyl Sulfoxide, 0.1M Sodium acetate trihydrate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K

Cysteine-ligand loop, Hydrolase, OXIDOREDUCTASE

MTTVVDRWNIHPDHLWLRGQRPESPVVFDETQGVWNVYGYPEAMDILNDHDTFTSDLAHLLPVSVDAPLLEGDMSQMDPP
RHRKYRQLVSRAFTPRLVADMETRVADITRELLDAVDGKPEIEIAADLAYPLPVIVIAELLGVPAGDRDLFKKWADDIIE
GFSGFSFLDTSGQGEQDVRDATERLRPLLDYMAGHVTERRRTPREDLLTHLVQAEVDGERLTDNEIVNVANILLVTGHIT
TTMTLGNTVLCLDADPEVAAKVRADRSLVPGAIEEALRVLSPSAALARGTSREVEVAGTVIPKDQIVMLWLGAGNRDPRQ
FTDPEVYDPTRDPNPHFGFGRGIHFCLGAPLARLEGRVALNALFDRFPVLRTDPAKPPTFFPTPDMIGVNTLHLRTS